The instrumental version of this song can be heard in the Pinkie Pie-themed levels of My Little Pony: Puzzle Party. It also serves as the second of two bonus tracks of side B on the Luna variant of the vinyl album Magical Friendship Tour and the second track on the My Little Pony 2015 Convention Collection. It is the third track of the Songs of Friendship and Magic soundtrack album, under the title The Smile Song. The song is also featured in Cakes for the Memories during reused footage from A Friend in Deed. Spike also mentions the Smile Song by name in Sweet and Smoky.

In Pinkie Pride, Cheese Sandwich sings a short polka rendition of the song during The Goof Off.

An instrumental version can be heard on the title screen for the My Little Pony iOS and Android game. Music from the song is used in the Pinkie Pie's Cupcake Maker game on the Hub Network's website. The song has been released as a sing-along on the DVDs My Little Pony Friendship is Magic: Pinkie Pie Party and My Little Pony Friendship is Magic: Season Two Disc 4 (where it is called 'Smile, Smile, Smile!').

The song was uploaded in mono sound to YouTube by user 'Veronica Dela Cruz' on 7 November 2011 but removed soon after the leak led to Hasbro 'cracking down' on material released by the show's artists.

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